The overexpression of Bra-miR07 can significantly influence the growth and development of transgenic
plants and Bra-miR07 may also play important roles in regulation of cauliflower
organ development.
In
the previous researches, researchers obtained a series of new miRNAs that
showed significantly different expression in the early organ development of
cauliflower by high-throughput sequencing. While in the present research, researchers
Zhang Qingli, Jin Chuan, Qin Erjun, Wang Yu Wu Mei, Li Lihong, Li Hui, Chen
Chengbin, Song Wenqin and Wang Chunguo further analyzed a new miRNA (named
Bra-miR07) that showed different expression between cauliflower hypocotyl and
cotyledon.
The results indicate that the sequence length of Bra-miR07 precursor is 270 bp
which can form the stable secondary hairpin structure. Bra-miR07 appears higher
expression in cauliflower cotyledon than that in hypocotyl. To further uncover
the function of Bra-miR07, researchers construct and transfer overexpression
vector of Bra-miR07 into Arabidopsis thaliana by floral dip, and successfully
obtain the Bra-miR07 overexpression transgenic plants. Phenotypic analysis
shows that overexpression of Bra-miR07 can significantly influence the growth
and development of transgenic plants. Compared with wild-type Arabidopsis,
Bra-miR07 transgenic plants show slow growth, dwarf plant and lower fertility.
Consequently, these results imply that Bra-miR07 may also play important roles
in regulation of cauliflower organ development.
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Tag: miRNA cauliflower