The researchers analyzed the sequence
variations of Pi d3 for rice blast resistance in 80 Yunnan rice landraces by
resequencing of open reading frame (ORF).
The results show that there are 39
nucleotide variations in 2775 bp ORFs of 80 rice accessions with an average
mutation rate of 1.41% compared with Pi-d3 Digu ORF. Based on nucleotide
variations, The WTBX Pi-d3ORF of 80 rice accessions can be divided into 37
haplotypes. Among these materials, H8 (28.8%), H4 (11.3%) and H23 (5.0%) are
high-frequency types while others belong to low frequency types, which shows
that the WTBX Pi-d3 in Yunnan rice landraces is rich in genetic variations and
haplotypes, but low in frequency.
There are 28 amino acids variations in 33
translation proteins of 37 haplotypes with a high frequency of pseudogene: 18
haplotypes with 32 rice landraces (include 12 indica rice varieties) are
examined to have Pi-d3 pseudogenes, and the reason may be the permeation of
Pi-d3 pseudogene to indica. Except for the published terminator at position
737, there is a new find that positions 32 and 467 of protein also have
terminator. Pi-d3 shows differences in the type and frequency of haplotypes
between the subspecies of indica and japonica, the ecotypes of lowland and upland
rice, non-waxy and waxy rice which shows that there is a genetic
differentiation in subspecies or ecotypes. The geographical distribution of 37
haplotypes has a characteristic of spreading over a large area while
concentrating in small areas, and Puer, Banna and Lincang are the central areas
with abundant haplotypes which means the types of haplotypes decreased outward
progressively.
*News from a paper titled Sequence
Variation of Pi-d3 for Rice Blast Resistance in Yunnan Rice Landrace delivered
byYang Yi, Sun Yiding, Ma Jiqiong, Wang Yanyan and Xu Minghui from the
Institute of Biotechnology and Genetic Resources/Key Laboratory of
Biotechnology Research of Yunnan Province/Key Laboratory of Southwestern Crop
Gene Resources and Germplasm Innovation of Ministry of Agriculture of Yunnan
Academy of Agricultural Sciences.
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